Evania Valensia Suhartono (2015) Efek Perendaman Resin Akrilik Dalam Ekstrak Buah Jeruk Nipis (Citrus Aurantifolia) Pada Pertumbuhan Candida Albicans. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background. Acrylic resin is a material that is often used in dentistry. The use of acrylic resin denture has the advantage also has the disadvantage that there is a micro- cavity attachment for debris that can occur plaque accumulation. Candida albicans is one of the normal flora in the oral cavity. Candida albicans as the main fungal species found in the denture wearing. The roughness of surface the more the accumulation of Candida albicans. In recent years, the increase of interest for knowing the properties of lemon that can affect the growth of Candida albicans. Purpose. To determine the minimal inhibitory concentration (MIC) of lemon fruit extract on the growth of Candida albicans on acrylic resin. Method. This research used a sample size of 1x1x1 mm acrylic resin, then the sample had been inserted into the tube containing the suspension Candida albicans. Then the sample was soaked in lemon fruit extract with a concentration of 20%, 25%, 30%, 35%, 40% and 2% ketoconazole. Each sample washed 2x with PBS solution and then inserted into Saboroud's broth 10 ml and vibrated for 30 seconds. Then planted in Saboroud's dextrose 37oC for 48 hours. after that, the colonies were counted. Result. The statistic results that counted by Independent t-test known significant difference (p <0.05). On the positive control was not obtained the growth of Candida albicans, whereas the lemon extract with a concentration of 20%, 25%, 30%, 35%, 40% found that the growth of Candida albicans 42.2000 CFU / ml, 33.2000 CFU / ml, 20.4000 CFU / ml, 14.0000 CFU / ml, 5.8000 CFU / ml. Conclusion. Based on the results of research, concluded that heat cured acrylic plate that soaked in 40% concentration of lemon extract effectively inhibit the growth of Candida albicans colonies due to the minimum growth of Candida albicans.

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