WIEKE SETIOWATI, 061011026 (2014) PROFIL BERAT MOLEKUL PROTEIN HASIL METABOLIT ISOLAT BAKTERI ASAM LAKTAT Pediococcus pentosaceus DENGAN METODE SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS. Skripsi thesis, UNIVERSITAS AIRLANGGA.

Preview	Text (ABSTRACT) gdlhub-gdl-s1-2014-setiowatiw-34480-4.abst-t.pdf Download (141kB) | Preview
	Text (FULLTEXT) gdlhub-gdl-s1-2014-setiowatiw-34480-1.FULLTEXT.pdf Restricted to Registered users only Download (984kB) | Request a copy

Abstract

The aim of this research is to get the protein profile based on protein molecular weight metabolite result isolate LAB Pediococcus pentosaceus by SDS-PAGE. The result of metabolite produces antibiotic-like compounds. Pediococcus pentosaceus can produces bacteriocin and utilization in biopreservation process. Before running SDS-PAGE, crude extract is purified. Purification in this research using ammonium sulfate precipitation with 20%, 40%, 60%, 80% and 100% saturation then continued dialysis process. The purification process is success at concentration 40% saturation, because the protein can be expressed the most compared to other concentrations. The result of protein profile from Pediococcus pentosaceus by SDS-PAGE have 17 bands with protein molecular weight 108.9 kDa, 92.9 kDa, 73.5 kDa, 67.6 kDa, 61.9 kDa, 56.5 kDa, 52.4 kDa, 46.9 kDa, 43.9 kDa, 39.4 kDa, 34.5 kDa, 31.9 kDa, 29.4 kDa, 27 kDa, 23.4 kDa and 14.2 kDa.

Actions (login required)

View Item