ARDILA MEGA PUSPITA, 060710207 (2011) PERBEDAAN WARNA KOLONI Salmonella pullorum dan Staphylococcus aureus PADA MEDIA EKSTRAK DAGING SAPI YANG DITAMBAHKAN SARI KACANG HIJAU, LAKTOSA dan PHENOL RED. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This research was aimed to investigate the differences between Salmonella pullorum and Staphylococcus aureus colonies in artificial medium that was made from beef extract, green bean juice, lactose and phenol red. The composition of media was 1.6 % agar base, 0.8 % beef extract, 20 % green bean juice, 1 % lactose, (0,08 g/l) phenol red solution and aquadest ad 1000 ml. The isolate of Salmonella pullorum from field and Staphylococcus aureus standart ATC 25923 were inoculated into the media with five repetitions. As control media that were made from beef extract, green bean juice, lactose and phenol red without inoculated by bacteria. Surrounding of Salmonella pullorum colonies seawed red and the changed its color to red, while surrounding of Staphylococcus aureus colonies changed its color to yellow gold. Analysis by chi-square show high significanty (p<0,01). Based on the result, this medium consist of beef extract, green bean juice, lactose and phenol red can be used as differential medium according to ability of bacteria in fermentating lactose by change of its color.

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