Apriantini, Nyta, NIM. 060012780 (2009) UJI KEPEKAAN BEBERAPA BAHAN ANTIMIKROBIAL TERHADAP Staphylococcus aureus PENYEBAB MASTITIS PADA SAPI PERAH DI SEKITAR KUTT SUKA MAKMUR GRATI PASURUAN. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this research is to know about sensitivity of Staphylococcus aureus that cause mastitis in dairy cattle around of Suka Makmur Dairy Cooperative, Grati. Antimicrobials that had been used was ampicillin 10 µg, carbenicillin 100 µg, ciprofloxacin 5 µg, gentamicin 10 µg, penicillin 10 unit, oxytetracycline 30 µg, and sulfametoxacol 25 µg by In Vitro using Kirby Bauer method. The quantity of Staphylococcus aureus that had been tested was 25 samples, that come from 268 cases mastitis milk samples and had been identified by macroscopic, microscopic, Gram colouring characteristics, and biochemical characteristics. The result of this research shows that 25 samples can be identified as Staphylococcus aureus that known by coccus, positive gram, positive catalase, positive reaction against mannitol, and positive coagulation. The sensitivity test of antimicrobial materials shows that oxytetracycline give the best results (100 %). The amount of samples are 92 % sensitive to gentamicin (n=23), 88 % sensitive to ciprofloxacin (n=22), 84 % sensitive to sulfametoxacol (n=21), and 80 % sensitive to (n=20). Just 52 % sensitive to penicillin (n=13) and 48 % sensitive to ampicillin (n=12).

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