WARDANI, KUSUMA EKA, NIM. 060213051 (2009) UJI KEPEKAAN BEBERAPA BAHAN ANTIMIKROBIAL TERHADAP Escherichia coli PENYEBAB MASTITIS PADA SAPI PERAH DI SEKITAR KUTT SUKA MAKMUR GRATI PASURUAN. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this study was to fmd out the sensitivity of several antimicrobial agents to Escherichia coli that caused mastitis in dairy cattle at around of Suka Makmur Dairy Cooperative Grati Pasuruan. Twenty five Escherichia coil isolates from milk samples of dairy cattle at around of Suka Makmur Dairy Cooperative which were showed the positive result of California Mastitis Test used in this study. The sensitivity test was carried out by Kirby-Bauer method. Antimicrobial agents to be tested were penicillin 10 IU, ampicillin 10 µg, oxytetracycline 30 µg, gentamicin 10 µg, ciprofloxacin 5 µg, carbenicillin 100 µg and sulfamethoxazol 25 µg. Disc of these antimicrobial agents were placed on the cultures of Escherichia coli at the surface of MHA media, incubated at 37° C for 24 hours. The diameter of inhibitor area around the disc, measured on mm and compared to the standart of NCCLS. The sensitivity test result that Escherichia coli were 100% sensitive to gentamicin and resistant to penicillin. The resistance grade of E.coli to the other antimicrobial agents followed by carbenicillin (52%), ampicillin, oxytetracycline and sulfamethoxazol (48%), and ciprofloxacin (12%).

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