ANDRIKA INDRA PRATOMO, 060610120 (2011) IDENTIFIKASI PROTEIN 36 kDa YANG DIDUGA KIT-LIGAND DARI CAIRAN FOLIKEL OVARIUM SAPI DENGAN METODE ELEKTROFORESIS. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The research was comprised of researches on protein Kit-Ligand in liquor folliculi ovary of bovine. Kit-Ligand was protein to improve the quality of oocytes for using in vitro embryo production. Kit-Ligand have to produced by granulose cells ovary follicle of bovine. Embryo production with in vitro method could use to Embryo Transfer technology. C-kit was receptor of Kit-Ligand . C-kit could be found in oocytes and granulose cells ovary of bovine. The research was aimed at identification of protein Kit-Ligand isolated from liquor folliculi of bovine ovary. Bovine ovary obtained from a slaughterhouse was aspired in its ovary follicle > 5 mm with spuit and needle then sentrifuged by sentrifugator. Liquor folliculi from sentrifuge method collected with pipet. Identification of protein Kit-Ligand was carried out by means of Sodium Sulphat Polyacrylamide Gel Electrophoresis (SDS-PAGE). From the result, several protein fractions were obtained. Based on the calculation of regression equation resulting from protein marker to determine the molecular weight of protein Kit-Ligand, 10 fractions were determined, 155,55 kDa, 87,28 kDa, 75,54 kDa, 63,31 kDa, 53,93 kDa, 48,98 kDa, 36,10 kDa, 26,10 kDa, 26,61 kDa, 22,67 kDa. Protein appearing in the protein band the molecular weight of 36,10 kDa was first identified as Kit-Ligand initiating follicle development.

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