Fatkur Fadholi, 060810288 (2012) DETEKSI ANTIBODI HOSPES PARATENIK (Mus musculus) YANG DIINFEKSI L2 Toxocara vitulorum DENGAN BERBAGAI DOSIS. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this research was investigated effect of various doses second stage larva of Toxocara vitulorum at paratenic host antibody toward Optical Density (OD) value using indirect ELISA technique. The research used twenty four male mices (Mus musculus) strain Balb/C and were divided into four groups. They were infected by second stage larva of T. vitulorum. The first group (P0) as control was not inoculated, the second (P1) was inoculated with dose 10 eggs/ mice, the third (P2) was inoculated with dose 5 eggs/g body weight, and fourth(P3) was inoculated with dose 10 eggs/g body weight. Fourteen days post infection, blood serum was taken as antibody. Antibody was reacted with antigen protein adult of T. vitulorum tissues and value was measured by using indirect-ELISA test. The data was analyzed by ANOVA and duncan 5% test. The result showed high difference significantly between the groups (P<0,01). It can be concluded that second stage larvae of T. vitulorum were immunogenic and produced different effect with various doses.

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