TRI SUSILO MARDI, 060610042 (2014) GAMBARAN RESISTENSI ANTIBIOTIKA ISOLAT Escherichia coli YANG DIISOLASI DARI DAGING SAPI YANG DIJUAL DI SUATU PASAR DI SURABAYA. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This study was aimed to determine the antibiotic susceptibility of E. coli bacteria isolated from beef sold at a wet market. Sixteen beef samples were bought from a wet market used in this study. The isolation of samples were used enrichment Brillian Greeen Bile Broth incubated at 370C for 24-48 hour, followed with plating on Eosin Methylen Blue Agar incubated at 370C for 24 hour. The suspect colony showed a metallic green colour was than inoculated in 1% Peptone Water Broth incubated at 370C for 24 hour for indol test. Six sample (37,5%) were confirmed as E. coli that also have characteristic of rod shape, gram negative. The antibiotic susceptibility of E. coli isolates were 100% sensitive to Oxytetracycline, but it were totally resistant to Ampicillin, Cloxacillin, Erytromycin and Penicillin.

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