Emi Diah P. S. P., 060513405 (2009) IDENTIFIKASI MATURTION PROMOTING FACTOR (MPF) DARI OOSIT KUMULUS KOMPLEK SAPI YANG DIMATURASI SECARA IN VITRO DENGAN METODE ELEKTROFORESIS. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This Study was a series of experiments on the Maturation Promoting Factor (MPF) protein fraction in the process of in vitro maturation for increasing the quality of oocytes in the in vitro embryo production. The aim of this experiment was to identify and characterize the Maturation Promoting Factor (MPF) protein fraction that isolated from mature oocytes of bovine ovaries from follicles with diameter 3 – 8 mm. The follicles of bovine ovaries obtained from slaughterhouse were aspired. Oocytes were lysed using sonication. Identification of Maturation Promoting Factor (MPF) protein fraction was performed with Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE) process. From the result of this experiment, several protein bands have appeared. Based of the calculation using regression equation on the protein marker for estimating the molecular weight of Maturation Promoting Factor (MPF), 8 protein fractions were obtained : 169.62 kDa; 135.39 kDa; 110.30 kDa; 98.08 kDa; 43.81 kDa; 32.25 kDa; 19.57 kDa; 12.85 kDa. Protein band with 32 kDa molecular weight was identified as Maturation Promoting Factor (MPF) that has the important role in the follicle development.

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