HENDRIK SETIA BUDI, 090810042 D (2013) EKSPRESI PDGF-BB, BMP-4 dan BMP-7SETELAH PEMBERIAN EKSTRAK GETAH BATANG PISANG AMBON (Musa paradisiaca var. sapientum) PADA PENYEMBUHAN LUKA PENCABUTAN GIGI Penelitian Eksperimental Pada Tikus putih (Rattus novergicus). Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Introduction : Blood clotting disturbance or excessive bleeding after tooth extraction, periodontal and implant surgery may affect to delayed of wound healing.Recently have been developed the use of PDGF and BMP growth factors therapy to accelerate wound healing such as in periodontal disease and implant placement, but the use of that material are very expensive. Ambon banana stem sap has been commonly used to accelerate wound healing and dental pain. Purpose : The purpose of this study was to elucidate the mechanism of wound healing after tooth extraction has given extract of ambon banana stem sap topically to the expression of PDGF-BB, BMP-4, BMP-7 as a mediator that plays a role in initiating the healing of the socket aveolar on fibroblast, osteoblast and osteoclast Methods : We have used the post-test only control-group design with several stages like phytochemistry screening, biocompatibility, stability of the drug in the form of gel, and the examination of the alveolar socket healing through immunohistochemical method to expression of PDGF-BB, BMP-4, BMP-7, and HE staining of fibroblast, osteoblast and osteoclast on 105 male rats devided to 5 groups. Tooth has extracted on right incisor of mandible and applicated topically to the socket that is 15 mg dose of ambon banana stem sap extract, 30 mg, 60 mg, gelatin sponge of hemostatic local as size 1X0,2x0,2 cm, and control group was given 4% HPMC. Immediately after surgery, the gingival tissues were sutured with monofilament, and irrigated by sterile distilled water every day. The animals were sacrificed 2, 7, and 14 days after tooth extraction by ether. The data were tabulated and analyzed statistically Result : The results showed that the active compounds of ambon banana stem sap contained saponin, flavonoids, tannins, anthraquinones and lectins. Biocompatibility test showed that the ambon banana stem sap at 10-100% concentrate was not toxic to cultured fibroblast. Ambon banana stem sap extract increase the expression of PDGF-BB, BMP-4 and BMP-7 depend of dose than 4% HPMC and gelatin sponge at 2, 7 and 14 days. The result of HE staining showed an increasing of fibroblast and osteoblast commencing at 2, 7 and 14 days, but showed inhibition to osteoclasts. There was dose relationship to PDGF-BB, BMP-4 and BMP-7 expression. The conclusion of this study : The conclusion of this study that the ambon banana stem sap extract could increase the expression of growth factors that play a role in initiating the wound healing of the socket aveolar by PDGF-BB, BMP-4 and BMP-7 expression indicator as biomarker in fibroblast, osteoblast and osteoclast cells. The optimal dose to accelerate the alveolar socket healing is 30 mg.

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