HERI SUROTO, 090710145D (2011) EFIKASI PENGGUNAAN KOMPOSIT FREEZE DRIED TENDON ALLOGRAFTDAN SEL PUNCA MESENSIMAL DALAM REKONSTRUKSI DEFEK TENDONFLEKSOR Penelitian Eksperimental Pada Hewan Coba. Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Flexor tendon defect caused by acute or chronic ruptured should be filled with tendon graft. So far there was no functional results with a tendon allograft obtained to address the flexor tendon defect. This study aimed to regain the integrity and biomechanical properties of tendons in the flexor tendon defect connected by composite of freezed dried tendon allograft and bone marrow mesenchymal stem cells. The design of this research was the randomized post test only control group. Experimental unit were male New Zealand White Rabbits. In these experimental animals were slaughtered their Flexor Carpi Ulnaris tendon right leg by 1 cm and then made a connection with reconstruction using autograft tendon (control group) and composite of freeze dried tendon allograft and bone marrow mesenchymal stem cells (treatment group). After 6 weeks FCU tendons that had been reconstructed were analyzed by macroscopic examination, histological, immunohistochemical, and biomechanical examination as well to assess the new tendon tissue regeneration. The Reconstructed flexor tendon defect with composite freeze dried tendon allograft –mesenchymal stem cell in cross sectional examination are bigger than autograft tendon (7,18 mm2 and 4,20 mm2 , p = 0,000).The Scaffold degradation process that expressed by MMP-8 (p=0,000) are more active, and also these happened in new tendon regeneration process through the expression of Scleraxis and Collagen type 1(p=0,000). Reconstruction of the flexor tendon defect with composite freeze dried tendon allograft – bone marrow mesenchymal stem cell actually decreased in maximal load (88%), tensile strength (78,7%), modulus elasticity (72,66%) compare to tendon autograft, but the difference is not significant. The conclusion of this research was composite freeze dried tendon allograft and mesenchymal stem cell can be used in tendon tissue engineering instead of autograft tendon.

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