EMMANUEL DJOKO POETRANTO (2012) KARAKTERISASI MOLEKULER DAN PROFIL PATOGENITASVIRUS H5N1 ISOLAT ASAL BURUNG GEREJA (PASSER MONTANUS)SEBAGAI SUMBER PENULARAN ALAMI DI INDONESIAPENELITIAN EKSPLORATIF DAN EKSPERIMENTAL LABORATORIS. Disertasi thesis, UNIVERSITAS AIRLANGGA.

Abstract

The isolation of an H5N1 Influenza A virus from a tree sparrow (Passer montanus) which we captured in East Java (Sidoarjo) was reported. The analysis showed that the virus titer was 10 6.5 Based on the result of phylogenetic tree and homology analysis revealed that its haemaglutinin (HA) and neuraminidase (NA) were genetically similar with the virus chiken isolated of East Java 2010 and the virus human isolated from Indonesia in 2006-2007. The sequence revealed that the HA protein cleavage site sequence was PQRESRRKKR and the sequence of receptor binding site was QSG. This showed that the virus is classified as High Pathogenic Avian Influenza H5N1, but it could not transmit among human. ELD 50 and the virus had antigenicity relationships with all the antisera panel derived from clade 2.1.3, but not with a panel of antisera derived from clade 2.1.1 that were considered representation of the virus in Indonesia. In the experimental laboratories, it was reported that the pathogenicity of this virus was higher in chicken than in tree sparrow. Thus tree sparrow was more potential to spread this virus than chicken. The finding of a tree sparrow H5N1 virus is genetically similar to the virus isolated from human, thus it is important to always monitor the existance of virus in wild birds to anticipate pandemic

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