RIESTA PRIMAHARINASTITI, 090610128 D (2011) PENGEMBANGAN METODE IDENTIFIKASI Salmonella typhimurium DALAM MAKANAN SECARA KROMATOGRAFI GAS-SPEKTROMETRI MASSA MENGGUNAKAN PROFIL ASAM LEMAK SELULER. Disertasi thesis, UNIVERSITAS AIRLANGGA.
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Abstract
The objective of the research was to construct a Principal Component Analysis (PCA) model based on cellular fatty acid profile of Salmonella typhimurium using GC-MS. The PCA model was used to discriminate Salmonella typhimurium among some foodborne pathogens such as Eschericia coli, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa. The principle of the technique is to analyze the whole fatty acid in bacterium, which is characterictic for each species and exhibit the differentiation among them. The cellular fatty acid was determined by GC-MS using Fatty Acid Methyl Esther (FAME) method. The bacterium was incubated in CASO agar plate for 24 hours at 32°C prior methyl esterification process. The fatty acid profile then used to construct a PCA model for each bacterium. The result of this research showed that different species was able to be distinguished by the PCA model of each bacteria. The first two PC explained 71% of the total variance and together with the third PC, the model explained 94% of the variance. The first PC on the score-plot has separated the bacteria into two groups, based on the Gram-type of the bacterium. Salmonella typhimurium’s model was characterized mainly by the 9-hexadecenoic acid, an unsaturated straight chain fatty acid. This fatty acid is a marker of Gram negative bacterium. Experiment on calibration set and test set using PCA model of Salmonella typhimurium showed that Salmonella typhimurium in sample was able to be identified correctly. Identification of Salmonella typhimurium on food matrice using standard method and GC-MS method gave the same result. Using this cellular fatty acid PCA model, Salmonella typhimurium was succeed to discriminate from Eschericia coli, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa. This method has a potential capability as the detection method for Salmonella typhimurium contamination on food.
| Item Type: | Thesis (Disertasi) | |||||||||
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| Additional Information: | KKC KK Dis M 10/12 Pri p | |||||||||
| Uncontrolled Keywords: | cellular fatty acid, Salmonella typhimurium, GC-MS, PCA model, foodborne pathogen | |||||||||
| Subjects: | Q Science > QA Mathematics > QA16-20 Special Teaching Methods and Aids Q Science > QD Chemistry > QD241-441 Organic chemistry R Medicine > RA Public aspects of medicine > RA1-1270 Public aspects of medicine > RA421-790.95 Public health. Hygiene. Preventive medicine > RA601-602 Food and food supply in relation to public health |
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| Divisions: | 09. Sekolah Pasca Sarjana > Ilmu Matematika & IPA | |||||||||
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| Depositing User: | Nn Anisa Septiyo Ningtias | |||||||||
| Date Deposited: | 21 Jun 2017 22:02 | |||||||||
| Last Modified: | 21 Jun 2017 22:03 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/32809 | |||||||||
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