INYA RIDLO NIZOMY, 099913293M
(2002)
PENGARUH STRESOR EPINEFRIN TERHADAP PROLIFERASI OTOT POLOS PEMBULUH DARAH TIKUS PUTIH (Raitusnorvegicus)STRAIN WISTAR.
Thesis thesis, UNIVERSITAS AIRLANGGA.
Abstract
The research was conducted to investigate the effect of epinephrine (E) in vascular smooth muscle cells (VSMCs) proliferations in vivo using histomorphological femoral artery induced by artificial stressor (epinephrinedstressor). This posttest only control group design consists of 24 males Wistar rat weight 150 � 200 grams, divided into 2 groups for treatment and 1 control group for separate pretest. The I" treatment group were injected i.m with 0,125 ml E containing 0,05 mg.ml"' Epinephrine bitartras (ETHICA) per 200 grams (300 times dilution with Saline), and 2nd treatment group with Saline (OTSUKA Indonesia) for the same doses, twice a day at 7 AM and 7 PM. After 14 days treatment, the samples euthanazied with aether aenestheticus and femoral artery removed for histological cross luminal section preparation with Hematoksiline-Eosin dyes. The histomorphometry analysis i.e luminal area, medial thickness and VSMCs number (nuclei) counting were determined to established VSMCs proliferation. This analysis used Adobe Photoshop 5,0 (Adobe Inc. USA) computerized programe from scanned preparation's pictures callibrated with micrometer's objective glass (OB-µ -1/100 Olympus Japan) in the camera-lightmicroschope (Olympus-C-35AD-4 -Olympus BH-2; Japan). The experimental showed that there were increased of medial thickness and cell number between 2 groups of treatment 33,61 ± 5,12 µm and 157 ± 41 versus 63,33 ± 13,8 gm and 223 ± 45 respectively, but not for the luminal area. There were decreased of luminal area from Epinephrine group (8,548.104± 1,110 I.lm2) to Saline group (8,466. 104 t 3,262 µm2). Statistical multivariate analysis showed that there were significant difference in Hoteling's Trace value (p < 0,01) of three dependent variables for each treatment group. Continous analysis with univariate results significant difference (p < 0,01) for all dependent variables between and within group of treatment. Further analysis with discriminant analysis resulted 2 discriminant variables i.e luminal area and medial thickness (p < 0,05). After all, without ignoring any disturbing factors such as preparation technical false, these data suggest that we successfully prooved the VSMCs proliferation stimulated by epinephrined-stressor.
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