JULIANA CHRISTYANINGSIH, 090710144D (2011) PENGARUH SUPLEMENTASI VITAMIN C TERHADAP AKTIVITAS SITOKROM P450 1A1 (CYP1A1), GLUTATION-S-TRANSFERASE DALAM HEPAR DAN EMBRIOTOKSISITAS MENCIT DENGAN INTOKSIKASI TIMBAL. Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Lead contamination occurs through air pollution and industry, enter the body through respiratory and digestive tract. High lead content will accumulate and affect adversely the cognitive function, causing neuropsychological dysfunction, encephalopathy, hyperactivity and other problems in children, disrupt the central nervous system and the immune system of children as well. This experimental research is randomized control group post test only design. The experiment used of 27 pregnant mice, divided into three groups: negative control group, which were given distilled water, positive control group were exposed only to lead and the third group were exposed to lead and administered vitamin C. 25 mg / kg /day/ orally neutral lead acetate was given during gestation day 7 to 16, and vitamin C 64 mg / kg / day/ orally, started on gestation day 9 to 16. Treatment group with vitamin C supplementation has the lowest CYP1A1 and glutathione S-transferase enzyme activity compared to positive and negative control groups. This results confirmed by the molecular weight of CYP1A1 enzyme ranges 53.7 to 59.2 kDa, and the western blotting test showed the same thin band both two groups. The lowest of the average lead concentration in the head of fetal mice and the blood of mice were found on the group of mice that treated with vitamin C. There was no effect of lead exposure on the fetuses survival, but only decreased fetal body weight. Supplementation of vitamin C can protect the liver, and fetus, by suspected mechanism that vitamin C could chelate the lead and excrete it via urine.

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