Edi Suriaman, 081041019 (2012) EKSPLORASI DAN UJI POTENSI BAKTERI ENDOFIT DIAZOTROF PENGHASIL INDOLE ACETIC ACID (IAA) DARI BAWANG MERAH (Allium ascalonicum L.) KULTIVAR BIMA. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aims of this research to know abundace populatian and diversity of endophyte bacteria diazotroph (fixation nitrogen) from shallot plant (Allium ascalonicum L. cv. Bima), which can be producing indole acetic acid (IAA)compounds, and its potential to enhance the growth of shallot. This research is exploratory to detection total endophyte bacteria diazotroph in A. ascalonicum with Most Probable Number (MPN) method, and experimentally to determine the potential production of IAA by endophyte bacteria, and their potential in promoting growth shallot performed with 11 treatments and 6 replications. Observed variables for the characterization of endophyte bacteria diazotrof consist of morphology colony, cells bacteria and physiology. Observed variables to potential assay of IAA is the produced IAA concentration by bacteria at a variety of tryptophan concentration, as well as plant growth variables measured were root length, plant height, fresh weight (root and leaf), biomass (roots and leaves). The data were analyzed as descriptive quantitative and qualitative, and ANOVA with significance 5% and followed by Duncan. This research showed in A. ascalonicum L., contained endophyte bacteria diazotroph wich indicated by pellicle formation turns white on JNFb (James nitrogen free bromthymol blue) semi solid medium. Isolation showed there were 8 species of bacteria have been found that have similarity with A. paspali (97,2%), B. mycoides (1) (92,1%), P. pseudomallei (92,3%), B. mycoides (2) (94,4 %), B. mycoides (3) (93,6 %), P. cepacia (94,2%), Bacillus circulans (93,9%), dan B. Alvei (93,4 %). All species of endophyte bacteria diazotropht have the ability to produce IAA. The maximum concentration of IAA produced by each bacterial species is P. pseudomallei (14 mg/L), A. paspali (11,7 mg/L), B. alvei (9,38 mg/L), P. cepacia (9 mg/L), B. mycoides (3) (6,13), B. circulans (5,75), B. mycoides (2) (5,38 mg/L), and B. mycoides (1) (4,56). All endophyte bacteria diazotroph significantly to enhance the growth of shallot than control negative. A.paspali is bacteria that can increase most of shallot plant growth parameters significantly, as the length of the root is (15,117 ± 4,74 cm), the plant height is 26,22 ± 5,8 cm; fresh weight and root biomass each is 0,819 ± 0,33 dan 0,074 ± 0,02 g; as well as fresh weight and leaf biomass each is 3,815 ± 0,61 dan 0,243 ± 0,08 g.

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