IRINE NORMALINA, 090810532M (2011) STUDI PEMINDAHAN DNA PLASMID PENGKODE Extended Spectrum -lactamase (ESBL) DARI BAKTERI Escherichia coli ke Klebsiella pneumoniae MELALUI TRANSFORMASI DAN KONJUGASI. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Extended Spectrum -lactamase (ESBL) are enzymes produced by bacteria that play an important role in resistance to antibiotics, especially 1st, 2nd,and 3rd generation cephalosporin antibiotics. The spread of resistance among some bacteria, both in the same species and cross-species have increased. Presumably this increased resistance is caused by horizontal gene transfer through transformation and conjugation. To prove the existence of horizontal gene transfer, in this research, the process of transfer of plasmid DNA from E. coli ESBL positive to K. pneumoniae ESBL negative. The result is, the transfer of plasmid DNA from E. coli ESBL positive to K. pneumoniae ESBL negative work done through the mechanism of transformation marked by expressed gene encoding a positive ESBL in K. pneumoniae and caused K. pneumoniae become resistant. Meanwhile, through the mechanism of conjugation, gene encoding a positive ESBL successfully transferred into K. pneumoniae and caused K. pneumoniae resistant to cefotaxime and aztreonam, and are sensitive to ceftriaxone and ceftazidime, but resistance to cefotaxime and aztreonam are not classified as ESBL. It is thought to be caused by back mutation in the sequence composition, or changes in the structure of ESBL enzymes.

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