IDA BAGUS RAI WIADNYA, 080942010 (2012) PENGEMBANGAN METODE ANALISIS MELAMIN DALAM SUSU BERBASIS REAKSI DIAZOTASI MENGGUNAKAN - NAFTOL. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

A spectrophotometric method development's has been investigated for the determination of melamine levels in milk. This method is based on the presence of primary aromatic amine on the melamine to be reacts with nitrite acid to form diazonium salt. Diazonium salt was then coupled with β-naphthol reagent to form a colored azo compound. Absorbance was measured at a maximum wavelength of 552 nm. In this method, optimization was done for reagents acidity, concentration and incubation time for maximum formation of azo dyes. Optimization results were obtained by concentration of HCl 0,25 M, NaNO2 0,008 M, β-naphthol 0,0008 M and the incubation time of 7 minutes. Standard deviation, coefficient of variation, limits of detection and limit of quantification of the results were obtained 0,00632, 2,75%, 9,09.10-6 M and 3,03.10-5 M with a sensitivity of 1139 L / mol, and the linearity 0,986 respectively. Percent recovery was obtained for melamine in milk with a concentration of 4.0 10-5 M, 7,2. 10-5 M, and 2,0. 10-4 M is 103,31%. Percent recovery of standards melamine with concentrations of 2,4. 10-5 M, 5,6. 10-5 M, and 8,0. 10-5 M is 95,27%. This method has been applied to the analysis of melamine in milk.

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