SUPATMI, 090710286 M
(2009)
EFEK 2-METHOXYETHANOL TERHADAP KADAR TESTOSTERON DANHISTOLOGIS TESTIS MENCIT (MUS MUSCULUS L).
Thesis thesis, UNIVERSITAS AIRLANGGA.
Abstract
2-Methoxyetbanol (2-ME) is one of chemical agents that is derived from the metabolite of dimethoxyetilphatalate (DMEP) from the group phtalic acid ester (PAEs) used as plastizier in producing plastics. The compound 2-ME given to experimental anima1s were found to produce toxicity in reproductive system and the testis as the primary target organs, and may lead to the damage of spermatogenic cells (spermatogenesis) and the steroidogenetic process as welL 1he objective of this study was to test testoterone concentration and the histology of the testis (the number of spermatogonoia, primary spermatocyte, and oval spermatid) that was given with 2-ME through subcutaneous injection in a varied dose. This study used complete randomized design. The experimental unit in this stu4y comprised adu1t ma1e Balb/c strain mice (Mus musculus), aged 6-8 weeks , with body weight of 28-30 g. The anima1s were classified into 3 groups, each group comprising 10 mice. Two groups served as treatment groups, receiving 2ME of 50 and 100 mg/kg BW, and one group served as control receiving subcutaneous 0.90.4. NaCI for 12 days. Resu1ts showed significant reduction in testosterone concentration with p == 0.002, and, regarding testicu1ar histology, the number of spermatogonia and primary spermatocyte showed significant reduction (p = 0.008 and p = 0.050), while spemtatid count increased with p 0.86, with a conclusion that 2-ME reduces testosterone concentration as well as the number of spermatogonia and primary spermatocyte.
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