Dewi Ratna Sari, NIM011141019 (2014) PENGARUH DOSIS VITAMIN D (CHOLECALCIFEROL) TERHADAP KADAR GULA DARAH PUASA (GDP), DIAMETER ADIPOSIT SERTA EKSPRESI GLUCOSE TRANSPORTER (GLUT) 4 DI ADIPOSIT TIKUS DENGAN DIABETES MELLITUS Penelitian Eksperimental Laboratoris pada Tikus Putih (Rattus norvegicus) Jantan Strain Wistar. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Vitamin D may have a role in modifying the risk of diabetes. However, studies on the mechanism and dose exploration of the vitamin D is still unclear and requires further investigation. The objective of this study was to examine the effect of vitamin D administration in different doses on fasting blood glucose (FBG) level, the diameter of adipocyte and the expression of GLUT 4 in adipocyte of diabetic rats. Twenty eight male rats (Rattus norvegicus) of Wistar strain were fed high fat diet for 5 weeks and a single intraperitoneal injection of streptozocin (35 mg/kg) at the end of the 2nd week was administered to induce diabetes mellitus. Diabetic rats were divided into group K, group X1, group X2, and group X3. Vitamin D were administered with different doses 6,25 g/kg in X1, 12,5 g/kg in X2 and 25 g/kg in X3 per oral, once, everyday for 14 days. Twenty four hour from the last treatment, FBG level was measured from the blood vein tail, then the animals were sacrified. Subcutaneous adipose tissues were processed into histological preparation. Data were analyzed with one way ANOVA test (= 0.05). There were no significance different of FBG level (p=0.199) and diameter of adipocyte (p=0.218) between groups. There was significance different of GLUT 4 expression in adipocyte (p=0.035) between groups, where the differences were found between group K and groups X1, X2, and X3. Vitamin D administration with different doses can improve the expression of GLUT 4 in adipocyte without followed by alteration of FBG level and diameter of adipocyte of diabetic rats.

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