ELLY NURUS SAKINAH, NIM011141024 (2013) EFEK PEMBERIAN CHOLECALCIFEROL TERHADAP TRANSLOKASI PROTEIN GLUT4 SEL OTOT RANGKA PADA MENCIT HIPERGLIKEMIA HASIL INDUKSI STREPTOZOTOCIN. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Diabetes mellitus is a global health burden because the everincreasing incidence. Glucose uptake into skeletal muscle cells require insulindependent and insulin independent signaling pathways, both leading to the translocation of glucose transporter-4 (GLUT4) to the plasma membrane. Insulin resistance occurs due to failure of insulin signaling to translocate GLUT4 resulting in the failure of glucose uptake and causing hyperglycemia. Numerous studies have emphasized that increase in the amount of GLUT4 is a potential target for therapeutic of hyperglycemia. Cholecalciferol is known to have a function in regulating calcium homeostasis was shown to increase the synthesis of insulin, and increase GLUT4 translocation through an alternative pathway that is independent of insulin signaling pathway. The purpose of this study is to explain the role of cholecalciferol to increased insulin sensitivity in hyperglycemia mice models through increased GLUT4 translocation in skeletal muscle cells. 30 mice adapted for one week and then induced using 150mg/kgBW streptozotocin(STZ) intraperitoneally, After experiencing hyperglycemia mice were divided into 5 groups (n=6 each), Group I (hyperglycemic control), group II (25ng cholecalciferol), group III (50ng cholecalciferol), group IV (100ng cholecalciferol), and group V (metformin 300mg/kgBBB). Cholecalciferol given orally for 14th days. On day 15th the examination of fasting blood sugar levels measured using a glucometer and the mice were sacrificed so then the gastrocnemius muscle tissue can be taken for immunohistochemical examination using rat GLUT4 polyclonal antibodies. The assesment of protein GLUT4 was conducted by quantifying Immuno Reactive Score (IRS-GLUT4). Based on statistical analysis showed that there were significant differences in fasting blood sugar levels between groups (p<0,001) also found differences in the number of cells of skeletal muscle GLUT4 translocation between groups (p<0,001). Based on univariate regression analyses there was significant doses response relationship (p<0,001). Cholecalciferol may lower fasting blood sugar levels and increase protein GLUT4 expression in skeletal muscle cell membranes in hyperglycemia mice models.

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