HEBERT ADRIANTO, NIM011214353001 (2014) AKTIVITAS BIOLARVASIDA EKSTRAK DAUN Citrus spp. DAN Pandanus amaryllifolius TERHADAP STADIUM LARVA Aedes aegypti DENGAN PENDEKATAN BIOSISTEMATIKA NUMERIK. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aims of this study are firstly to know biolarvicidal toxicity value (LC90) from leaf extracts of Citrus spp. and Pandanus amaryllifolius against Aedes aegypti, secondly to find out the prime species and alternative species as biolarvicidal, thirdly analyze their relationship, and finally to identify the similarity between the prime species and alternative species and their position in their relationship. This study was conducted using nested experiment design. The biolarvicidal activities, phytochemical screening, and relationship in biosistematic numeric of five plant, Citrus hystrix, Citrus aurantifolia, Citrus amblycarpa, Citrus maxima, and Pandanus amaryllifolius have been evaluated in this study. Methanol leaf extract of Citrus spp. and Pandanus amaryllifolius were tested at 500, 1375, 2250, 3125, and 4000 ppm concentrations against the 3rd instar larvae of Aedes aegypti. Larvae mortality was observed after 24 hours and data were analyzed by probit. Morphology and anatomy diversity of five plant were analyzed by hierarchial cluster and PCA (Principal Component Analyses). The results of this study show that Citrus hystrix leaf extract has the highest toxity and the most effective among others with LC90=2.828,249 ppm, followed by Citrus aurantifolia and Citrus amblycarpa. Biosistematic numeric shows that Citrus hystrix clustered with Citrus aurantifolia at 61,8% similarity value, followed by Citrus amblycarpa at 48,3% similarity value and Citrus maxima at 44,3% similarity value, in addition Pandanus amaryllifolius made a single cluster. Therefore there is similarity between the prime species (Citrus hystrix) and alternative species (Citrus aurantifolia) with their position in their relationship shown in biosistematic numeric.

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