RADITYO PRADIPTA, 091214353003 (2014) PENGARUH IMUNOTERAPI EKSTRAK TERIPANG (Paracaudina australis) TERHADAP KADAR IFN- , dan GAMBARAN HISTOPATOLOGI PARU PADA TIKUS (Rattus norvegicus) MODEL TUBERKULOSIS. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this research was to know the immunotherapy effect of sea cucumber Paracaudina australis,based on IFN- levels and histopatological view of the lung on murine model tuberculosis. This research used 24 male rat, Wistar strain, with age range 8-10 weeks and average weight 200 g. Paracaudina australis were collected from east coast Surabaya and being extracted using ethanol. Mycobacterium tuberculosis strain H37RV 105 per mL were used as agent of infection. Rats were grouped into four groups; K- negative control group were not given sea cucumber's extract nor infected with M.tb; K+ positive control group were not given cucumber�s extract but infected with M.tb; T1 were given sea cucumber's extract with dose 15mg/200grBW and infected with M.tb; and T2 were given sea cucumber's extract with dose 30mg/200grBW and infected with M.tb. After infected by M.tb for 3 weeks, T1 and T2 were given Sea cucumber's extract continously for 2 weeks using gavage method. After that, termination for each group were held, followed with IFN- levels and lung's histopatology examination. In the histopathology feature, we done semi-quantitative procedure to measure lung damage by using Dormans score method. The obtained data then analyzed with oneway Anova (=0,05), followed with Duncan test. As the result, it can be confirmed that Paracaudina australis extract with dose 30mg/200grBW had immunotherapy effect by affecting the level of IFN- (most high) and histopathological view of lung (less damage) on murine model tuberculosis

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