YETI EKA SISPITA SARI (2015) BERCAK URIN UNTUK IDENTIFIKASI FORENSIK JENIS KELAMIN DENGAN LOKUS AMELOGENIN DAN Y KROMOSOM (DYS19). Thesis thesis, UNIVERSITAS AIRLANGGA.
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Abstract
Background: The location of amelogenin gene for identification of sex chromosome has good variability between the form and the shape of the X chromosome and the Y chromosome and between amelogenin alleles among different populations. Purpose: To prove urine spot examination on the results of the sex determination through Deoxyribo Nucleid Acid (DNA) isolation using amelogenin and Y chromosome loci (DYS19). Methods: Spotting the microscopic examination of urine samples to determine the presence or absence of urethral epithelial cells, followed by isolation Deoxyribo nucleid Acid (DNA) in order to determine the extent and purity of DNA amplification. Then performed Polymerase Chain Reaction (PCR) amelogenin locus at 106bp - 112bp and Y chromosomes (DYS19) at 232 -268 bp. Results: in 9 samples of men from 3 families with 3 kinship of different regions show the results of different test, because of Amel Y variation between individuals and populations method of determining the sex of 100% was inaccurate. In some men Amel Y can be removed entirely. This study should be visualized one band on the Y chromosome (DYS19) and the amelogenin two bands during electrophoresis occurs misidentification of the sample as a woman. Conclusions: Identification of sex using amelogenin locus and Y chromosomes (DYS19) has six identical and ambiguous results because the two samples shown as the sign of men but visualized as women, another sample was not visualized because of the thick level and concentration of Deoxyribo nucleid Acid (DNA).
Item Type: | Thesis (Thesis) | |||||||||
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Additional Information: | KKA KK TIF.01/15 Sar b | |||||||||
Uncontrolled Keywords: | Urine Spot, Sex Determination, Amelogenin, Y chromosome (DYS19) | |||||||||
Subjects: | R Medicine > RA Public aspects of medicine > RA1-1270 Public aspects of medicine > RA1001-1171 Forensic medicine. Medical jurisprudence. Legal medicine | |||||||||
Divisions: | 01. Fakultas Kedokteran > Ilmu Forensik | |||||||||
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Depositing User: | Nn Husnul Khotimah | |||||||||
Date Deposited: | 24 Oct 2016 22:41 | |||||||||
Last Modified: | 15 Apr 2020 11:56 | |||||||||
URI: | http://repository.unair.ac.id/id/eprint/39528 | |||||||||
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