ABDUL SAMIK (2011) EKSPLORASI PROTEIN SPESIFIK PREGNANCY ASSOCIATE!) GLYCOPROTEIN (PAG ) AIR SUSU SAPI PERAH Sebagai BIOMARKER UNTUK PENGEMBANGAN DIAGNOSIS KEBUNTINGAN SECARA LABORATORIS. UNIVERSITAS AIRLANGGA, Surabaya. (Unpublished)

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Abstract

The sustainability of livestock sources is rdatt:d to hirth rate increase and the shortening or kidding interval. One of the elTorts to shorten the calving interval in dairy cattle is hy conducting early diagnosis of prcgnanc: alter mating. The development of early pregnancy diagnosis method in livcskx:k can he undertakell h~ observing specific substance rresenls in the milk of pregnant livestock. i.c. the Pregnancy Associated Glycoprotein (PAG). The o~jective of this study was to prove that PAG protein was able to induce humoral immune response characterizcu by: the fonnation of anti-PAG and to prove that anti-PAG produced was able to recognize PAG protein from milk of pregnant dairy cow as the base Il)r developing OIlC orearly pregnancy test in dairy cow. This study was conducted by following these stages: I) Characterization or anti-PAG produced from the induction of PAG protein isolate from the milk of pregnant dairy cow and 2) Dairy cow pregnancy diagnosis by PAG microtiter strip and rectal palpation. Anti-PAG titer obtained in this study was found to be low in week 5 after the first and second booster (week 10). i.e .• 44.76 !1g/m1 and 69.76 Jlg/ml, while the highest titer was obtained in week 3 after the first and second booster (week 8), i.e .. 555.00 Jlg/ml and 606.91 ~tg/ml. The results of PAG microtiter strip at 21th day after breeding wilh recti!! palpation revealed sensitivity, specificity. accuracy. positive predictive. and negative predictive values of 39,39 %; 80 %; 58 92,86 % and 44A4 %. 'hi1c tht.: results or 28th PAG microtiter strip at day after breeding with rectal palpation f'Cc'calcd sensitivity, specificity, accuracy, positive predictive. and negative predictive values 01'93,93 %; 85,71 %; 86 %; 86,11 % and 85,71 Based on the results, it can be concluded that PAG protein is able to '1I1duce humoral immune response by the fonnation of anti-PAG. The anti-PAG produced can be employed for doe pregnancy diagnosis with PAG microliter strip. Dairy cOVv pregnancy diagnosis with PAG microtiter strip at 281h day after hrccding has more reliable validation value as compared at 21th day after breeding.

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