BIAN DWI CAHYO, 051211131165 (2016) UJI AKTIVITAS ANTIMIKROBA FRAKSI-FRAKSI DARI EKSTRAK ETIL ASETAT JAMUR ENDOFIT Penicillium oxalicum YANG DIISOLASI DARI SPONS Homaxinella sp. Skripsi thesis, Universitas Airlangga.

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Abstract

Penicillium oxalicum is one of endophytic fungus isolated from sponge Homaxinella sp.. Previous study showed that ethyl acetat extract of P. oxalicum posses antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Bacillus subtillis. The purpose of the current study is to determine the antimicrobial activity of fractions from ethyl acetat extract of P. oxalicum. The assay was conducted by using disc diffusion method. The results showed that fractions 2-4 and 6-8 with concentration 100 μg/disc had antimicrobial activity against Escherichia coli ATCC 8739 with inhibition zone 8.2 – 9.3 mm. Fractions 2-5 and 7-9 with concentration 100 μg/disc were able to provide antimicrobial activity against Staphylococcus aureus ATCC 6538 with inhibition zone 7.3 – 8.9 mm. Fractions 2-8 with concentration 100 μg/disc gave antimicrobial activity against Bacillus subtillis ATCC 6633 with inhibition zone 8.5 – 10.2 mm. Keywords : Antimicrobial activity, fractions, endophytic fungi, P. oxalicum, sponge Homaxinella sp.

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