FRENBY PERDANA, 051211133020
(2016)
PENETAPAN KADAR PESTISIDA SIPERMETRIN DALAM DAUN TEH HIJAU (Camellia sinensis) DENGAN METODE KCKT.
Skripsi thesis, Universitas Airlangga.
Abstract
DETERMINATION OF CYPERMETHRIN PESTICIDES IN
GREEN TEA LEAVES (Camellian sinensis ) WITH HIGH
PERFORMANCE LIQUID CHROMATOGRAPHY
Frenby Perdana
Teas are often disturbed by pest, especially the plant ones.
Cypermethrine is one of the insecticide that used widely among
botanists to control mites. Besides that, cypermethrine is effective
againts variety of pest. Pesticide is a substance or material that has a
great potential danger to environment and health. Then, the
consentration of cypermethrin were determined using high performance
liquid chromatography (HPLC) method. Chromatographic separation
were achieved with μbondapak C18 (3.9 x 300)mm 10μm 125A column
and the mobile phase were a mixture of acetonitrile:water (80:20). The
retention time of cypermethrin was 6.020 min, and the flow rate were
0.75 mL/min. The UV detector wavelength were set on 230 nm. The
method was validated for: specificity, linearity, accuracy, precision,
limits of detection, and limits of quantification. The method was proved
to be selective. Linearity showed linear respone (r = 0.9995) while
correlation coeffecient of the function (Vxo)was 2.06 % over the range of
concentration used (between 0.3776 mg L-1 to 3.7757 mg L-1). Accuracy
of method was in range of accepted limits (80% - 120%), % recovery
average 101.26%. The detection limit is 0.1140 ppm and the
quantification limit is 0.3455 ppm. The result of the analysis are %
cypermethrin consentration on day 0 is 2,86x10-2±1,10x10-4, on day 1
is 2,50x10-2±1,10x10-4, on day 2 is 8,45x10-2±5,00x10-5, on day 4 is
4,13x10-3±1,30x10-4, on day 8 is 5,83x10-4±2,00x10-5 and on day 10 is
3,86x10-4±3,00x10-5. The conclusion of this proven that any changes
that were happened in the day, can reduce levels cypermethrin.
Keyword : Pesticide cypermethrin, green tea leaf, high performance
liquid chromatography.
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