BAGUS SRIAJI, 051211131164 (2016) AKTIVITAS ENZIM FIBRINOLITIK Bacillus pumilus T4 ISOLAT TERASI SIDOARJO PADA MEDIA BUNGKIL KEDELAI. Skripsi thesis, Universitas Airlangga.

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Abstract

FIBRINOLYTIC ENZYME ACTIVITY OF Bacillus pumilus T4 ISOLATED FROM TERASI SIDOARJO ON SOYBEAN MEAL MEDIA BAGUS SRIAJI Fibrinolytic enzyme can be isolated from many sources, especially microorganisms. Microorganism genus have been known their strong fibrinolytic activity was Bacillus, which isolated from fermented food. One of Indonesia traditional fermented food is terasi. Previous research isolated Bacillus pumilus which exhibited a fibrinolytic activity from terasi Sidoarjo. The component of growth media may affect the fibrinolityc activity of Bacillus pumilus of terasi Sidoarjo. Therefore, the aim of this study is to find out the effect of soybean meal concentrations to the fibrinolityc enzyme activity. The qualitative test found that the Bacillus pumilus T4 from terasi Sidoarjo has been showed proteolytic and fibrinolity activities. The Proteolytic and fibrinolytic index was 2,83 ±1,02 and 1.84 ± 0.15 respectively. The maximum optical density (λ 580) 0,722, 1.040, 0.915, 1.152, and 1.193 after 24, 33, 36, 30 and 36 hours respectively were achieved at 1.0%, 1.5%, 2.0%, 2.5% and 3.0% of soybean meal media. The maximum fibrinolytic activity 0.3207 U.mL-1.min-1, 0.2225 U.mL-1.min-1, 0.4653 U.mL-1.min-1, 0.3642 U.mL-1.min-1, and 0.4097 U.mL-1.min-1 after 30, 33, 33, 33, and 33 hours respectively were achieved at 1.0%, 1.5%, 2.0%, 2.5%, and 3.0% of soybean meal media. Keywords : terasi, fibrinolytic, Bacillus pumilus, soybean meal

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