Kamadjaja, Josef and Rostiny and Amalia, Fitri Rizki (2012) Efektivitas ekstrak biji jintan hitam (Nigella sativa) dalam menghambat pertumbuhan mikroorganisme rongga mulut pada cetakan polyvinyl siloxane. Journal of Prosthodontics, 3 (1). pp. 23-28. ISSN 2087-1856

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Abstract

Background. Impression materials can be one of the oral cavity microorganisms transmission media. To prevent this, polyvinyl siloxane impression needs to be disinfected. Purpose. The aim of this research is to find out the effective concentration of black cumin seed (Nigella sativa) extract in inhibiting the multiplication oral microorganism on polyvinyl siloxane impession. Method. The research on the growth of oral microorganism was done using 7 samples. Polyvinyl siloxane impression samples that got from responden were cut at the first upper molar by a ring with 1 cm diameter that divide into 4 parts. Soaking for 10 minutes done in regards to the concentration of black cumin seed (Nigella sativa) extract respectively 12,5%, 25% and 50% and the control (sterile distilled water). Then inserted into the media (BHIB) with dilution until the tird tube. Then do the seeding of 0.1 ml from BHIB on blood agar media. The plates incubated for 24 hours and then the collonies of microorganism were counted. Data were analyzed by Kruskal-Wallis test and Mann-Whitney test. Result. There are significant differences of oral microorganism colonies embedded in polyvinyl siloxane after being soaked in sterile distilled water as a control and after soaking with black cumin seed (Nigella sativa) extract a concentration of 12.5%, 25% and 50%. Conclusion. The higher concentration of black cumin seed (Nigella sativa) that are used, the less the number of colonies of Streptococcus mutans.

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