ERRY TRI SHELIANA ADIKARA, 061141010 (2016) PENGARUH PENAMBAHAN CRUDE PROTEIN PLASMA SEMEN SAPI DALAM PENGENCER SUSU KUNING TELUR SEMEN DOMBA EKOR GEMUK TERHADAP MOTILITAS, VIABILITAS, FRAGMENTASI DNA DAN NEKROSIS SETELAH EQUILIBRASI. Thesis thesis, Universitas Airlangga.

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Abstract

The aim of this research was to determine the effects of crude protein bovine seminal plasma addition in skim milik and egg yolk diluent in increased motility and viability as well as decreased of DNA fragementation and necrosis of ram spermatozoa (Domba Ekor Gemuk). This study was used Complete Random Design with six replications and three treatments. Control treatment (P0) is consist (ram semen + diluent); treatment I consist of (ram semen + diluent) crude protein bovine seminal plasma in the 1:1 proportion; treatment II consist of (ram semen + diluent) crude protein bovine seminal plasma in the 1:2 proportion, then stored at 5°C. All treatments examined after 1 hour after equilibration. The results showed that addition of crude protein bovine seminal plasma (1:1 proportion) in skim milk and egg yolk diluent gave siginificant effects on motility, viability, DNA fragmentation, and necrosis of ram spermatozoa, whereas addition of crude protein bovine seminal plasma (1:2 proportion) had cause law motility, more proteins binding with sperm membrane will restricts movement of spermatozoa.

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