RANI WILUJENG ASMOROWATI, 061311133043 (2017) REAKSI SILANG PROTEIN Haemonchus contortus DENGAN SERUM ANTI-L2 Toxocara vitulorum MENGGUNAKAN TEKNIK WESTERN BLOT. Skripsi thesis, Universitas Airlangga.

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Abstract

The purpose of this research was to know cross reaction between protein Haemonchus contortus with serum anti-L2 Toxocara vitulorum using western blot technicque. The protein cross reaction can’t be used as a haemonchosis serologic diagnostic because make false positif with toxocariasis diagnostic. The Toxocara vitulorum worms collected form intestine of cattle and Haemonchus contortus worms collected from abomasum goats. The first step is make antibodies by oral infection infective eggs (L2) Toxocara vitulorum in mices. Take the blood twenty-one days after infection, then centrifuge the blood 1500 rpm 10 minutes to get serum. The second step, make homogenates from Whole Worm Extract (WWE) Haemonchus contortus. After crushed the worms, centrifuge 5000 rpm 15 minutes and take the supernatant. The supernatant then analysis using Sodium Dodecyl Sulphate Polycramide Gel Electrophoresis (SDS-PAGE) with comassie brilliant blue staining. The third step is anaysis Haemonchus contortus protein with serum anti-L2 Toxocara vitulorum using western blot technique. The result of H._contortus homogenates analysis using SDS-PAGE obtained 16 protein bands. The result of analysis western blot obtained 6 protein bands cross reaction are 141,3; 81,3; 64,6; 51,3; 46,8 dan 38 kDa.

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