Roedi Irawan and Subijanto Marto Sudarmo and Suhartono Taat Putra and Soetjipto (2012) Efek Pemberian Glutamin terhadap Aktivitas Sucrase, Maltase, Lactase dan Ekspresi Spectrin, Clathrin dalam Perbaikan Mikrovili Ileum pada Tikus Malnutrisi (The Effects of Glutamine on Sucrase, Maltase, Lactase Activity and Spectrin, Clathrin Expression in Repairing Microvilli Ileum in Rats Malnutrition). Jurnal Biosains Pascasarjana, 14 (1). pp. 48-60. ISSN 1412-1433

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Abstract

Glutamine is the most abundant amino acid in the blood and plays a key role of intestinal response to local and systemic injuries such as diarrhea and malnutrition. The purpose of this study was to explain the effect of oral glutamine on sucrase, maltase, lactase enzyme activity and spectrin, clathrin expression also the correlation with ERK-1 and STAT-3 signaling path ways in repairing microvilli ileum intestine in rat malnutrition. The method employed in this study was experimental research, with control samples, measura ble and reliable, with the design of the study was post test only group design. showed that twenty growing male rats (aged 12 week) were divided into 4 groups: control, malnutrition, control+glutamine and malnutrition+glutamine. For the first 15 days experiment, animals in the test groups received a karak diet (low calorie diet of dried rice) for the next 15 days. Control and malnutrition groups received either glutamine supplement with the doses 500 mg/kg body weight/day. On the 30th days, the animals were weighed and sacriced of the intestine was taken and repared for enzymatic and expression examination. Sucrase activ ity between malnutrition with malnutrition+glutamine groups there indicated significance ileum (p = 0.04). Maltase activity when compared malnutrition with malnutrition + glutamine groups there were significant differences in the ileum (p = 0.00). Lactase activity when compared between malnutrition with malnutrition + glutamine groups there were signi  cant differences in ileum (p = 0.04). The expression of spectrin and clathrin were evidence enlarge of enterocyte in the normal group after being given glutamine com pared to malnutrition group after being fed glutamine, spectrin and clathrin expression appearance on ileum. Spectrin and clathrin molecular weight protein in the ileum with a molecular mass of 130-250 KDa. Relationship between glutamine and meaningful pathway signaling proteins ERK-1 in the ileum (p = 0.03) and also significant association between glutamine and STAT-3 in ileum (p = 0.00). The research proved that there was an increase activity of sucrase, maltase, lactase, also spectrin and clathrin which have expression differences of each gro up. There are links between ERK-1 and STAT-3 signaling pathway in the repairing microvilli of rat ileum intest inal damaged by malnutrition and improved after administration of oral glutamine

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