Prahasanti, Chiquita (2013) IMMUNOHISTOCHEMICAL ANALYSIS OF NF-kB (P50/P65 ) IN PATIENT WITH AGGRESSIVE AND CHRONIC PERIODONTITIS. Indonesian Journal of Tropical and Infectious Disease, 4 (4). pp. 59-64. ISSN 2085-1103

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Abstract

Background: Nuclear factor-kappaB (NF-κB) is a protein complex that plays a role in transcription factors and in response to inflammation. Periodontitis is a periodontal disorder caused by various bacteria such as A. actinomycetemcomitan and P.gingivalis whose LPS is closely related to NF-κB (p50/p65). Aim: This study observed whether NF-κB (p50/p65) played a role in aggressive and chronic periodontitis. Methods: Data were obtained from periodontal tissue 40 patients with aggressive periodontitis and 40 patients with chronic periodontitis. Samples were derived from periodontal tissue with abnormalities and NF-κB (p50/p65) protein expression test was performed by immunohistochemistry. The statistical test used was the t-test. Results: In NF-κB (p50) the t value was -12 041 and significance 0.000, with α = 5%, showing significant difference in protein expression of NF-κB (p50) between patients with aggressive periodontitis and chronic periodontitis. OR estimation for the value of protein expression of NF-κB (p50)was 0.64 (sign. = 0.000). It shows that if the protein expression of NF-κB (p50) of the respondents is incremented by 1 (one) unit, the risk of chronic periodontitis increases 1.64 times. Box plot diagram shows that the distribution of the protein expression of NF- κB (p50) between patients with aggressive periodontitis and chronic periodontitis patients is significantly different. In NF-κB (p65) the Z value was -7.137 and significance of 0000, with α = 5%, showed significant differences in protein expression of NF-κB (p65) between patients with aggressive periodontitis and chronic periodontitis. OR estimates for protein expression of NF-κB (p65) was 0.66 (sign. = 0.000). This indicates that if the protein expression of NF-κB (p65) respondents is incremented by 1 (one) unit, the risk of chronic periodontitis increases 1.5 times. Box plot diagram shows that the distribution of the protein expression of NF-κB (p65) between patients with aggressive and chronic periodontitis patients is significantly different. Conclusion: The protein expression of NF-κB (p50/p65) has more influence on the incidence of chronic periodontitis patients, so it can be used as a marker for chronic periodontitis. Key words: periodontitis, inflammation, NF-κB p50, NF-κB p65, transcription

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