FIRDHAUSYAH NURAINI, 051311133101 (2017) UJI TOKSISITAS IN VITRO INFUSA DAN EKSTRAK ETANOL 70% DAUN UNGU (Graptophyllum pictum (L.) Griff.) PADA SEL DARAH MERAH MENGGUNAKAN SPEKTROFOTOMETER UV-VIS. Skripsi thesis, Universitas Airlangga.

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Abstract

Graptophyllum pictum leaves are traditionally used for the haemorrhoid. The objective of this research is to determine the interaction of the infusion and 70% ethanolic extract of Graptophyllum pictum leaves with human red blood cells using spectrophotometer UVVis. The toxicity of the infusion and 70% ethanolic extract of Graptophyllum pictum leaves in human red blood cells was measured by in vitro hemolysis tests. The absorbance of hemoglobin release was read by spectrophotometer UV-Vis at 576 nm. The infusion and 70% ethanolic extract of Graptophyllum pictum leaves had no toxicity effect on human red blood cells at 1 – 1,000 ppm. The hemolysis percentage of the infusion and 70% ethanolic extract of Graptophyllum pictum leaves at 1,000 ppm are 6.281% and 5.292% respectively. The hemolysis percentage of both samples are < 10%. The value of HC50 can not be determined. Based on phytochemical screening test, alkaloid presence in 70% ethanolic extract but absence in the infusion. The conclusion of this research is the infusion and 70% ethanolic extract of Graptophyllum pictum leaves are not toxic.

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