GRACE FRANSISKA, 051311133142 (2017) PENETAPAN KADAR TOTAL D-GLUKOSA DAN D-MANOSA YANG DIHITUNG SEBAGAI GLUKOMANAN PADA SERBUK PORANG (Amorphophallus oncophyllus) DAN SERBUK KONJAK (Amorphophallus konjac) DENGAN METODE SPEKTROFOTOMETRI VISIBEL. Skripsi thesis, Universitas Airlangga.

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Abstract

Porang/konjac is one type of tubers that are widely used in industry, food, cosmetics, and medical. That is because porang/konjac contains glucomannan, a polysaccharide composed of D-glucose and D-mannose. One way to determine the content is by using phenol-concentrated sulfuric acid reagent with Visible Spectrophotometric method. Before determinate how much glucomannan in the sample, the color stability test and method validation were performed. From the color stability test, the absorbance was observed after 180 minutes. For the validation result the selected wavelength was 492,0 nm, the equation of regression was y = 0,0266x + 0,0019 (r = 0,9992 > r table = 0,834) and Vxo 2,16%. While the precision gave the coefficient of variation 0,54% and accuracy gave average recovery for porang powder (99,60 ± 0,86) % (w/w) and (100,48 ± 0,56) % (w/w) for konjac powder. The determination of glucomannan was performed and the result showed that the concentration of glucomannan in porang powder was (52,33 ± 0,74) % (w/w) and for konjac powder was (61,24 ± 0,60) % (w/w).

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