Khoirotin nisak, 051414153004 (2017) PENGARUH VANADIL SULFAT TERHADAP PROLIFERASI SEL β PANKREAS PADA MENCIT DIABETES YANG DIINDUKSI STREPTOZOTOCIN PENELITIAN EKSPERIMENTAL LABORATORIS. Thesis thesis, Universitas Airlangga.

Preview	Text (abstrak) abstrak.pdf Download (85kB) | Preview
	Text (full text) thesis full.compressed.pdf Restricted to Registered users only until 13 November 2020. Download (1MB)

Abstract

This study aimed to determine the effects of vanadyl sulfate to stimulate proliferation of pancreatic β cells and characterized by an increased number of islet of Langerhans, β cell number and the number of Ki-67 expression. DM animal model was done by using male BALB/C mice induced by streptozotocin (ip) dose of 100 mg/kgBW followed by 50 mg/kgBW. DM achieved when blood glucose levels> 300 mg / dL. Animals were then grouped into 5 groups: normal group, DM group, and treatment group of vanadyl sulfate (po) dose of 5 mg/kgBW, 30 mg/kgBW, or 100 mg/kgBW (n = 6) for 7 days. Animal then sacrificed, stained, and scored by Allred method. Statistical result by using One- Way Anova showed that there is significant differences in blood glucose levels (p = 0,0129), only between normal group and DM group. There is no significant difference for the number of islet of Langerhans between groups. For the number of β cells showed there is significant difference (p <0,05) between groups with an increase in the number of β cells along with increasing doses of vanadyl sulfat. While the number of Ki-67 expression showed no significant difference between groups. it can be conclude that vanadyl sulfate may improve pancreatic β cell proliferation, but without an increase in the number of islets of Langerhans and increased expression of Ki-67. The mechanism of how the proliferation of pancreatic β cells still needed a further research

Actions (login required)

View Item