MICHAEL RAHARJA GANI, 051414153024 (2017) PENGEMBANGAN DAN VALIDASI METODE KCKT UNTUK ANALISIS MORAKHALKON A DALAM PLASMA DARAH KELINCI IN VITRO. Thesis thesis, Universitas Airlangga.

Preview	Text (abstrak) abstrak.pdf Download (239kB) | Preview
	Text (full text) Tesis Michael Raharja Gani.pdf Restricted to Registered users only until 13 November 2020. Download (2MB)

Abstract

Artocarpus champeden ethanol extract has been reported as antimalarial and prospect to developed as phytomedicine products which morachalcone A was determined as it’s active marker compund. To support the development of phytomedicine products from Artocarpus champeden especially in bioavailability and clinical study, a selective and sensitive analytical method becomes important for the determination of morachalcone A in blood plasma. The aim of this study was to develop and validate selectivity and sensitivity of HPLC method for determination of morachalcone A in rabbit plasma. This method was performed using a RP-C18 Column (250 x 4.6 mm i.d., 5 μm parcticle size), under isocratic elution with acetonitrile : water (50:50 v/v), detection was carried out at 368 nm and analyses were run at a flow rate of 1,0 mL/min, 4-hydroxychalcone and methanol were used as internal standard and precipitate agent. The results showed that this method was selective and good linearity in range of 3096,774-154.839 ng/mL for morachalcone A in rabbit plasma. LOD and LLOQ were found 89.384 and 154.839 ng/mL, respectively. The mean difference method was found between 2,79-14.33%. The intra-day and inter-day precision were both lower than 15% and recovery from extraction method from morachalcone A and Internal Standard were 80-120%.

Actions (login required)

View Item