NINDIA PUTRI TRININGTYA, 061324253006 (2017) PROFIL RESISTEN ANTIBIOTIK SERTA DETEKSI GEN PENGKODE ereA TERHADAP ISOLAT Escherichia coli DARI SUSU SEGAR DI WILAYAH JAWA TIMUR. Thesis thesis, Universitas Airlangga.

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Abstract

One bacteria that should not be in milk is Escherichia coli. The discovery of pathogenic bacteria or commensal in milk resistant to antibiotics has significance effect for public health. The aim of this research is to know the resistance and find fragment of resistance gene of Escherichia coli bacteria against antibiotics. Sample was taken from 5 dairy farms in East Java. It was tests for CMT, isolation and Escherichia coli identification, sensitivity test, and PCR test. The results showed that from 150 milk samples, there were 128 (85,33%) mastitis samples and 94 samples were identified Escherichia coli. of 94 isolates Escherichia coli 100% resistant erythromycin; 100% sensitive chloramphenicol; 53.8% isolate N, 65.4% isolate G, 73.3% isolate B, and 68% isolate S sensitive to oxytetracycline; 46.1% isolates N and G, 36.8% isolate B, and 52% isolate S showed intermediate for gentamycin. The PCR result test showed 2 positive samples from 12 isolates which resistant erythromycin in the amplification of the ereA gene primer. It is necessary to test the other encoding genes against erythromycin antibiotics.

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