Nikita Mundi, 061324253007 (2018) KARAKTERISASI PROFIL RESISTENSI ANTIBIOTIK PADA Escherichia coli YANG DIISOLASI DARI DAGING AYAM YANG DIJUAL DI BEBERAPA PASAR DI SURABAYA PENELITIAN EKSPLORATIF LABORATORIS. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Escherichia coli is a normal flora bacteria of the gastrointestinal tract of human and animals, but it can be pathogenic in certain circumstances due to the change of environment. E.coli as pathogenic bacteria causes diarrhea in human as well as in animals. This study aimed to know the antibiotic resistance profile of the E.coli isolated from chicken meat from several markets in Surabaya. The study was conducted using five antibiotics, which will be tested for its sensitivity to know the susceptibility level of E.coli against antibiotics. The study was done by bacteriogical and identification of 23 chicken drumstick sampels using Brilliant Green Bile Broth ( BGBB ), Eosyn Methylene Blue Agar ( EMBA ), Indol and biochemicals test. The E.coli identified isolates than tested for its sensitivity to five antibiotics sucs as Ampicillin, Chloramphenicol, Ciprofloxacin, Erythromycin and Penicillin. Polimerase Chain Reaction (PCR) examination was done to idenfy gen shv. The result of study showed that 19 (82.6%) isolates out of 23 sampels were identified as E.coli. The profile of antibiotics resistance of E.coli isolates were 19 (100%) samples resistant to erythromycin. There were five (26%) resistant samples against chloramphenicol, 18 (95%) samples resistant to penicillin, five (26%) samples resistant to ciprofloxacin and nine (47%) samples resistant to ampicillin. Total of two E.coli has shv with length 450bp. It concluded that two out of 19 E.coli isolates were ESBL isolates having shv.

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