Rini Devijanti Ridwan (2009) The toxicity test of Streptococcus mutans 1 (c) 67 kDa monoclonal antibodies in cell culture with MTT assay = Uji toksisitas antibodi monoklonal Streptococcus mutans 1 (c) 67 kDa pada kultur sel dengan MTT assay. Oral Biology Dental Journal, 1 (1). pp. 1-5. ISSN 1979-8814

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Abstract

Dental caries is infectious disease in hard tissue of tooth which cause dental structural disorder and chronic. From several the result of the study in Jakarta and Surabaya showed that the prevalency of dental caries was high. Therefore prevention of dental caries is still continuing, because the prevalency caries is high. There were many method to prevent dental caries, i.e. dental education, oral hygiene, special method on tooth brushing, water fluoridation, fissure sealant and later on the passive immunization with monoclonal antibodies. The material qualifications in dentistry must be non toxic, non iritation in tissue and have biocompatibility. The purpose of this study was to investigate about monoclonal antibodies toxicity of IgA, IgG1 and IgG3 in BHK-21 cell culture with MTT assay. A laboratory experimental study has been carried out. BHK-21 cell was included in microplate well with density 2×105 in 100 μl culture media, included for every well with IgA, IgG1 or IgG3 about 50 μl, appropriate with sample group. The cell control and media control were prepared too. Cell control was cell and culture media. Media control was only culture media. Replication for each monoclonal antibodies (with IgA, IgG1 and IgG3) was 10 times. Microplate with PBS (25 μl) and MTT 5 mg/ml was incubated for 24 hours in 37oC. Optical density formazan read by spectrophotometer with λ 540 nm. The data obtained in this study was analyzed with one way Anova and LSD. The result of this study showed that there was a significant difference of toxicity between IgA and IgG3 so IgG1 and IgG3. There wasn’t a significant difference of toxicity between IgA and IgG1 so between IgA with the control group, but there was a significant difference of toxicity between IgG1 and IgG3 with control group. The conclusion of this study showed that monoclonal antibodies of S. mutans 1 (c) 67 kDa wasn’t toxic and the material was safe to use with the lowest percentage level of the dead cell were IgA, IgG1 and IgG3.

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