IIN ELIANA TRIWAHYUNI, NIM011317017316 (2018) MEKANISME KEMOPREVENSI PERKEMBANGAN KARSINOMA SEL SKUAMOSA RONGGA MULUT MENCIT (Mus musculus) PADA PEMBERIAN EKSTRAK ETANOL BIJI KAKAO (Theobroma cacao) MELALUI PENINGKATAN IMUNITAS SELULER. Disertasi thesis, Universitas Airlangga.

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Abstract

Background:Head and neck cáncer ranks to six from all over the cáncer common in human. 48% of head and neck cáncer is in the oral cavity and 90% is oral squamous cell carcinoma. Cancer prevention can be done by chemoprevention. The control of transformed cell growth can be done by using polyphenol which is one of the cocoa (Theobroma cacao) content. Cellular immunity plays a role in the control of growth and development of transformated cells. Objective: to prove cocoa beans ethanol extract can increase IFNγ ,CD95L, grandzyme B, caspase-8, apoptosis, and decrease Ki67, and to clarify the mechanism of OSCC chemoprevention by cocoa beans ethanol extract. Material and methods: This research used 20 Balb/c mice devided into 5 groups. K0= normal control, K1= negative control, K2= given benzopyrene and 4mg/30BW/po/day extract, K3= given benzopyrene and 8mg/30gBW/po/day extract, K4= given benzopyrene and 16mg/30gBW/po/day extract. Cheek mucosa were biopsied and be used to IFN γ, CD 95L, grandzyme B, caspase-8, apoptosis, and Ki 67 expression examination by immunohistochemistry and tunnel assay. Results: there were significant differences on IFNγ (p=0.002), CD 95L (p = 0.000) , grandzyme B (p = 0.000), caspase-8 (p = 0.002), apoptosis (p = 0,018), and ki 67 (p = 0.002). Conclution, these data indicated that the ethanol extracts of cocoa beans could increase the expression of IFNγ, CD 95L, grandzyme B, caspase-8, and the number of apoptosis, as well as lowering the expression of Ki67. The mechanism of OSCC chemoprevention were through perforin/grandzyme pathway of apoptotic by increasing of CD 95L and Grandzyme B and through decreased expression of Ki67 directly.

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