Ernie Maduratna, Setiawatie and Noer Ulfah, Ulfah and Dian Agustin, Wahjuningrum and Desi Sandra, Sari and M Rubianto, Rubianto (2017) Viability Bovine Tooth Hydroxiapatite on Bone Marrow Mesenchymal Stem Cells. In: Proceedings of International Medical Device and Technology Conference (iMEDiTEC 2017). Medical Device and Technology Group, Johor Bahru, Johor, Malaysia on 6 – 7 September 2017. ISBN 978-967-0194-93-6 FBME

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Abstract

Different types of bone-graft substitutes have been developed. Hydroxyapatite is a scaffold- has the ability to perform osteoinduction and osteoconduction activity. Of these, Bovine tooth hydroxyapatite (BTHA) is a novel material produced by Airlangga University. Bone marrow mesenchymal stem cells (BMSCs) is a potential material in the regenerative medicine. It has tissue source and multipotent somatic stem cells. BMSCs have been combined with biomaterials such as osteoinductive and osteoconductive scaffold in order to improve bone regeneration. This study was performed to determine the viability of BTHA on bone marrow stem cells. This study is an experimental research. BTHA was extracted from bovine's teeth and formed into particles 355-710 μm. The viability effect of BTHA scaffold on BMSCs in a variety concentration 10%,50% and 100% (w/v)were analyzed by using 3-(4,5'dimethylihiazol-2-yl),2.5-di-phenyl-tetrazolium bromide (MTT) assay. Results were analyzed with ANOVA to see the difference between group. The highest viability of the cells (97.1%)were found in concentration 10% by using MTT assay. Viability BTHA 10% and 50 % on BMSCs is not different significantly (p≤ 0.05). BTHA is viable in 10% and 50% on bone marrow mesenchymal stem cell. In addition, economical production of BTHA should be taken into consideration. In future, BTHA may be a viable alternative for bone grafting when clinical trials have been completed.

Item Type:	Book Section
Subjects:	R Medicine R Medicine > RK Dentistry
Divisions:	02. Fakultas Kedokteran Gigi > Periodontics
Creators:	Creators NIM Ernie Maduratna, Setiawatie UNSPECIFIED Noer Ulfah, Ulfah UNSPECIFIED Dian Agustin, Wahjuningrum UNSPECIFIED Desi Sandra, Sari UNSPECIFIED M Rubianto, Rubianto UNSPECIFIED
Depositing User:	Rudy Febiyanto
Date Deposited:	18 Jul 2018 17:44
Last Modified:	18 Jul 2018 17:44
URI:	http://repository.unair.ac.id/id/eprint/73081
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