BUDIATIN, ANIEK SETIYA and ZAINUDDIN, MUHAMMAD and KHOTIB, JUNAIDI (2014) Biocompatible composite as gentamicin delivery system for osteomyelitis and bone regeneration. International Journal of Pharmacy and Pharmaceutical Sciences, 6 (3). p. 4. ISSN 0975-1491

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Abstract

Objective. The present study was designed to investigate the potency of BHA-GEL-GA-GEN pellet as gentamicin (GEN) delivery system matrix which has been cross-linked with glutaraldehyde (GA) 0.5%. Methods. The composition of Bovine hydroxyapatite (BHA) compared to the composition of Gelatin (GEL) is 10 : 1. Pellets are cylindrical with 4.0 mm diameter and weighed 100.0 mg. GEN releasing is evaluated by in vitro and in vivo examinations based on the concentration of GEN in 28 days. Results. Based on in vitro examination, it indicates 86% of GEN are released gradually in 28 days. Meanwhile, in vivo examination by implanting the pellet on rabbit femur indicates C max of GEN release on proximal area reached 24.32 g/L in 14 days and 26.97 g/L in 28 days for distal area. X-ray examination indicates the pellets have circular form as its defects at the second day. At the 28 th day examination, the pellets have transformed into compact form which filled the defects. This result indicates the pellets have been degraded and compatible with surrounding tissue. GEL is a type-1 collagen which is the main component of softcallus (osteoid). It undergoes calcification and transformed into hardcallus (osteocyte). High availability of calcium provided by BHA as delivery system accelerated this remodeling process. Conclusion. It can be concluded that in 28 days BHA-GEL-GA-GEN as local delivery system releases higher concentration of GEN compared to MIC Staphyllococcus aureus. BHA and GEL serve as bone remodeling template in osteomyelitis treatment. Keyword: Bovine Hydroxyapatite (BHA); Gelatin (GEL); Gentamisin (GEN); Cross-link, Glutaraldehyde (GA); Drug Delivery System (DDS).

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