RIFKA ANGGRAINI ANGGAI, 051524153009 (2018) PENGARUH KONSENTRASI KITOSAN DAN TRIPOLIFOSFAT TERHADAP KARAKTERISTIK FISIK DAN PELEPASAN BOVINE SERUM ALBUMIN DARI SISTEM MIKROSFER INHALASI. Thesis thesis, Universitas Airlangga.

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Abstract

Protein are unstable molecules because of their complex structure with large molecular weights and easily degraded by enzymatic systems. Bovine serum albumin (BSA) is commonly used as a protein model because BSA is biocompatible, non-toxic and non-immunogenic. Biodegradable microspheres have been used in protein delivery system. In this study, pulmonary protein delivery system was chosen as an alternative to targeted the protein. Chitosan/tripolyphosphate microspheres have already been used to encapsulated BSA. The aim of this work was to microencapsulate these protein-CS/TPP by ionic gelation and freeze-drying. BSA microspheres were produced with the ratio of chitosan:TPP. The morphology, particle size, moisture content, FTIR, thermal analysis, drug content, encapsulation efficiency and aerodynamic diameter of the prepared microspheres were investigated. The results revealed that the microspheres exhibited good sphericity and smooth surface area, microsphere ranged in size from 2.25 to 2.73 μm, that smaller particles are formed. The FTIR and DTA measurements have been performed to study interaction between the chitosan and TPP. The study found that the encapsulation efficiency of BSA in CS-TPP microspheres ranged from 88.14% to 94.80%. The deposition of microspheres particles in the stage of cascade impactors were determined and resulted many particles deposited in stage with diameter > 9 μm. The results suggested that iontoropic gelation method will be an effective method for fabricating chitosan microspheres for delivery of protein.

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