SHARA JAYANTI, 061614453001 (2018) AKTIVITAS ANTIKANKER DARI NANOPARTIKEL EKSTRAK BAWANG BOMBAY (Allium cepa. L) SEBAGAI INHIBITOR TERHADAP SEL MCF-7 SECARA IN VITRO PENELITIAN EKSPERIMENTAL LABORATORIS. Thesis thesis, Universitas Airlangga.
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Abstract
The purpose of this research was to manufacture nanoparticles from Allium cepa. L extract which containing flavonoids, saponins and tannins that can inhibit the proliferations of MCF-7 cells in vitro which tested by IHC to know supresses of Bcl2 protein expression and activated Caspase-3 protein. The research samples were nanoparticles from Allium cepa. L extract and MCF-7 cells in vitro. Nanoparticles was making done by SEM and PSA test. Nanoparticles extract fromAllium cepa. L tested by the KLT test to see the active ingredients and the result show that there are flavonoids, saponins and tannins in the nanoparticles extract from Allium cepa. L. Treatment nanoparticles from Allium cepa. L extract was administered to MCF-7 cells at doses of 40 μg/ml, 80 μg/ml, 160 μg/ml and 320 μg/ml. Treatment Allium cepa. L extract was administered to MCF-7 cells at doses of 40 μg/ml, 80 μg/ml, 160 μg/ml and 320 μg/ml. The result showed that nanoparticles extract fromAllium cepa. L has cytotoxicity to MCF-7 with IC50 was 235 μg/ml and extract from Allium cepa. L has cytotoxicity to MCF-7 with IC50 was 319 μg/ml. This samples was tested by IHC assay to see the expression levelof Bcl-2 protein and expression level of caspase- 3. The result showed that nanoparticles from Allium cepa. L extract was more suppress the expression of Bcl-2 protein than Allium cepa. L extract that showed by color change the cytoplasmic cell to be blue after stained by HE and nano particles from Allium cepa. L exstract can activate caspase-3 protein more than Allium cepa. L extract that showed by color change the cytoplasmic cell to be brown after stained by HE.
| Item Type: | Thesis (Thesis) | |||||||||
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| Additional Information: | KKC KK TVI. 06-18 Jay a | |||||||||
| Uncontrolled Keywords: | MCF-7, Allium cepa. L, Nanoparticles, Caspase-3, Bcl2 | |||||||||
| Subjects: | R Medicine > RA Public aspects of medicine > RA1-1270 Public aspects of medicine > RA421-790.95 Public health. Hygiene. Preventive medicine > RA440-440.87 Study and teaching. Research R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine |
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| Divisions: | 06. Fakultas Kedokteran Hewan | |||||||||
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| Depositing User: | Tatik Poedjijarti | |||||||||
| Date Deposited: | 23 Oct 2018 17:13 | |||||||||
| Last Modified: | 23 Oct 2018 17:13 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/75053 | |||||||||
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