NOVIANA ARIFIN, 061411131124 (2018) EFEK PEMBERIAN EKSTRAK BUAH OKRA (Abelmoschus esculentus) TERHADAP JUMLAH SEL SPERMATID DAN SEL SERTOLI PADA TESTIS MENCIT (Mus musculus). Skripsi thesis, Universitas Airlangga.

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Abstract

This research was aimed to determine the effect of Abelmoschus esculentus (okra) fruit extract towards spermatid and Sertoli cells number on Mus musculus testicle. This study used 20 male mice with an average weight of 25-30 grams. These animals were divided into four groups randomly (P-, P1, P2 and P3). P- (control) was treated with sterile aquadest without adding Abelmoschus esculentus fruit extract. P1, P2, and P3 administered orally 200, 400, 800 mg/kg BW/day of okra extract. On the 52nd day, the mice were euthanized by cervical dislocation. The spermatid and Sertoli cells could be observed by utilizing 400x microscope magnification. The result was analyzed with Analysis of Variance (ANOVA) and followed by Duncan’s Multiple Range Test (DMRT). The research result demonstrated that the Abelmoschus esculentus extract addition could decrease the number of spermatid siginificantly (p≤0,05) (P1 86.260±0.9290, P2 74.200±1.3285, P3 50.460±2.43) and decrease the number of Sertoli cells significantly (P1 8.800±0.4690, P2 7.040±0.2191, P3 8.655±0.3114). It could be concluded that each dose of the treatment of okra fruit extract could decrease the number of spermatid and Sertoli cells.

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