NADYA AMBARWATI, 051411131070 (2018) PEMURNIAN DAN KARAKTERISASI METABOLIT FRAKSI- 5 EKSTRAK ETIL ASETAT JAMUR ENDOFIT Aspergillus salwaensis DTO297C1 YANG DIISOLASI DARI TUMBUHAN Chromolaena odorata. Skripsi thesis, Universitas Airlangga.

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Abstract

Endophytic microbes is microbes that live in plant tissues without endangering their hosts. Endophytes produce some substances that have biological activity. Aspergilus salwaensis DTO297C1 is one of the endophytic fungi isolated from Chromolaena odorata. It is prove that 7 of 18 ethyl acetate fractions have an inhibiting activity of Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, and Candida albicans ATCC 10231. The objective of this study was to purify and characterize metabolite from fraction-5. Purification using column chromatography with silica gel 60 and n-hexane : ethyl acetate-methanol 20% gradient eluent. It is obtained 400 vial that combined and become 9 subfractions, 5.1 – 5.9. 5.1 and 5.6 subfractions was analyzed using Thin Layer Chromatography with silica gel 60 F254 as stationary phase and Chloroform:methanol (9,5:0,5) as mobile phase. 5.1 and 5.6 subfractions have active compound in UV 254 scan, positive in H2SO4 anisaldehyde stain removal, and 5.1 subfraction positive in FeCl3. 5.1 and 5.6 subfractions analyzed using GC-FID and GCMS. 5.1 shows predicted compound 2,4-Dihydroxy-3-methylacetophenone and 5.6 shows predicted compound Alpha-Terpinolene dan Camphor.

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