LAILI IRFANAH, 051411131008 (2018) PERBANDINGAN METODE DIFUSI AGAR DAN DILUSI SECARA TURBIDIMETRI UNTUK UJI DAYA HAMBAT MADU RANDU TERHADAP PERTUMBUHAN Bacillus subtilis ATCC 6633. Skripsi thesis, Universitas Airlangga.

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Abstract

Honey was known to have antibacterial activity against Gram positive and Gram negative bacteria. Agar diffusion and turbidimetry were used to antibacterial activity test for honey. However, there is still no report on which of these two methods is better for determination of antibacterial activity for honey. This study attempts to compare the agar diffusion and turbidimetric method for growth inhibitory activity test of ceiba honey against Bacillus subtilis ATCC 6633 based on some validation parameters including Limit of Detection (LOD), linearity, precision, and selectivity. The sample was ceiba honey which was made as 20% until 100% aqueous solutions. It was found that the minimum inhibitory concentration (MIC) considered as LOD of agar diffusion and turbidimetric methods were 35% and 30% respectively. Plot of inhibitory zone diameter against log honey concentration in agar diffusion method yields linear regression equation having r= 0.9804 and Vx0=1.06%, while r and Vx0 from plot of % transmittance against log concentration in the turbidimetric method were 0.9748 and 1.24% respectively. The coefficient of variation (CV) for agar diffusion method were 1.78% (repeatability) and 3.13% (intermediate precision) whereas for turbidimetry were 3.64% and 4.05% respectively. Agar diffusion and turbidimetric methods were selective because they can give the different response of ceiba honey from the different place and negative control. It can be concluded that the agar diffusion method and turbidimetry are valid and suitable for growth inhibitory activity test of ceiba honey against Bacillus subtilis ATCC 6633 and there was no significant differences between these methods. The turbidimetry has the advantage of being more sensitive because its lower LOD and the experimental technique is more simple than the agar diffusion method.

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