SITI HAJARIYAH FAHYUNA, 061411133063 (2018) IDENTIFICATION OF AVIAN INFLUENZA VIRUS SUBTYPE H9 AT CULLING LAYER IN INDONESIA. Skripsi thesis, Universitas Airlangga.

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Abstract

The aims of this research are to identify Avian Influenza Virus subtype H9 from culling layer in Indonesia and to know the percentage of this virus found based on the positive sample that collected from trachea and cloaca swab. The 350 samples of trachea and cloaca swabs from culling layer were examined by isolating on embryonated SAN chicken egg followed by Hemagglutination and Hemagglutination Inhibition using antisera H9 test. The research was conducted at Professor Nidom Foundation laboratory at Surabaya, inside the Biosafety Cabinet (BSC). The surveillance started in January 2018 and the location of sampling was taken from DKI Jakarta, West Java, Yogyakarta, East Java and South Kalimantan at year 2012 throughout 2018. The 350 samples were collected which is 10 samples each year for each regency. The result from Hemagglutinin Inhibition using antisera H9 showed, 3 positive sample in year 2012 (1.7%), 0 samples in year 2013 throughout 2015 (0%), 16 sample from in year 2016 (4.5%), 0 sample in year 2017 (0%) and 3 samples in year 2018 (1.7%) were positive, indicating the presence of potential Avian Influenza virus subtype H9 in culling layer.

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