MARIA LILIANA SANTOSO, 021618036310 (2018) DEGRADASI EXTRACELLULER POLYMERIC SUBSTANCE (EPS) BIOFILM ENTEROCOCCUS FAECALIS AKIBAT LAMA PAPARAN LASER DIODE 405 NM. Thesis thesis, Universitas Airlangga.

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Abstract

Background: Enteroccouss faecalis anaerob facultative gram positive bacteria. Enterococcus faecalis is a microorganism commonly detected in asymptomatic, persistent endodontic infections. Biofilm formation is defense mechanism of bacteria, main composition of biofilm is extraceluller polymeric substance (EPS). Photodynamic therapy is an alternative to eliminate bacterial biofilm. Purpose: This study aimed to determined the degradation of E. faecalis’s EPS using photodynamic therapy with 405nm diode laser and photosensitizer chlorophyll. Method: This study used E.faecalis, diode laser 405nm as a light source and chlorophyll as photosensitizer. Five different groups were analyzed: control group, chlorophyll group, chlorophyll + laser 90”, chlorophyll + laser 105”, and chlorophyll + laser 120”. The EPS was determined using Confocal Laser Scanning Microscope (CLSM). Result: Irradiation duration affects degradation of EPS. Group chlorophyll + laser 120” showed significant EPS degradation compared to other groups (p<0.05). Conclusion: Photodymanic theraphy using laser diode 405nm and fotosensitizer chlorophyll 120” could degrade Enterococcus faecalis’s EPS.

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